SiELC Biokolonner - Nukleotider

Primesep 200 för Nukleotidbaser

Primesep 200 är en Reversed Phase-kolonn med svagt sura joniska grupper embedded i Reversed Phase-kedjan.

HPLC Application for Separation of Nucleotide Bases
HPLC Application for Separation of Nucleotide Bases

Nucleotide bases are parts of DNA and RNA. Adenine and guanine are purine-bases; uracil, thymine and cytosine are pyrimidine-bases. In the view of chromatography these compounds are very polar and similar in properties. It is hard to obtain base line separation on traditional C18 as peaks of nucleotide bases co-elute even at low organic concentration. In this application nucleobases are well retained and separated on Primesep 200 mixed-mode column. Compounds are retained by weak reverse phase and weak ion-exchange mechanisms. Method can utilize UV, ELSD, and LC/MS detection.

Primesep SB Mixed-Mode för Nukleotidfosfater

Primesep SB är en Reversed Phase-kolonn med starka basiska joniska grupper embedded i Reversed Phase-kedjan.

Separation of Nine Nucleotides by Mixed-Mode Chromatography
Separation of Nine Nucleotides by Mixed-Mode Chromatography

Applikation – Separation of Nine Nucleotides by Mixed-Mode Chromatograpy

Monofosfater

Separation of Nucleotide Monophosphates
Separation of Nucleotide Monophosphates

Nucleotides are the monomers of DNA and RNA, composed of a five-carbon sugar, a nitrogenous base, and at least one phosphate group. Primesep SB is a good column for separating these highly polar compounds. Primesep SB is a reverse-phase column with strong embedded basic ion-pairing groups. Retention can me manipulated by adjusting acetonitrile, and the baseline separation can be achieved in under 6 minutes using a gradient. 



Sara Johansson
Applikationskemist
031 336 90 12
sara@scantecnordic.se



Mattias Arvidsson
Applikationskemist
031 336 90 06
mattias@scantecnordic.se